期刊
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
卷 119, 期 -, 页码 80-87出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbb.2013.11.014
关键词
Methamphetamine; Oxytocin; Medial prefrontal cortex; Dorsal hippocampus; Reinstatement; Restraint stress
资金
- National Key Scientific Project for New Drug Discovery and Development, PR China [2010ZX09401]
- Key Laboratory for New Drug Screening
- Key Laboratory for Pharmacodynamics of Liaoning Province, PR China
Our previous study revealed that intracerebroventricular oxytocin (OT) markedly inhibited the restraint stress-priming conditioned place preference (CPP) reinstatement induced by methamphetamine (MAP) via the glutamatergic system. In this study, the effect of microinjection with OT into mesocorticolimbic regions, the medial prefrontal cortex (mPFC) and the dorsal hippocampus (DHC), on the restraint stress-priming CPP reinstatement were further studied. The results showed that a 15-min restraint stress significantly reinstated MAP-induced CPP, which was inhibited by the microinjection of OT (0.5 and 2.5 mu g/mu l/mouse) into the mPFC. Atosiban (Ato), a selective inhibitor of OT receptor, could absolutely block the effect of OT (2.5 mu g/mu l/mouse). The reinstatement was inhibited by microinjecting with OT (2.5 but not 0.5 mu g/mu l/mouse) into the DHC, which could not be reversed by Ato. Western blotting results showed that the levels of GLT1, VGLUT2, NR2B, p-ERK1/2 and p-CREB expressions in the mPFC were increased and CaMKII was decreased markedly after the stress-priming MAP-induced CPP reinstatement test. OT blocked the changing levels of GLT1, VGLUT2, NR2B, p-CREB and CaMK II, which were reversed by Ato, but failed to affect the elevated expression of p-ERK1/2. In DHC, the levels of VGLUT2, p-ERK1/2 and CREB expressions were reduced during the stress-induced reinstatement, which could be reversed by OT and further abolished by Ato. The present results suggest that mPFC and DHC play differential roles in restraint stress-priming CPP reinstatement induced by MAP and OT via OT receptor affects the reinstatement in which the glutamatergic system is involved. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
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