期刊
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
卷 99, 期 1, 页码 94-99出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbb.2011.04.009
关键词
Nociception; Morphine; Sleep deprivation; Tyrosine hydroxylase
资金
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ)
- Universidade do Estado do Rio de Janeiro
Paradoxical sleep deprivation (PSD) increases pain sensitivity and reduces morphine antinociception. Because dopaminergic neurons in the periaqueductal gray matter (PAG) participate in pain modulation and opioid-induced antinociception, we evaluated the effects of PSD on thermal pain sensitivity, morphine- and L-DOPA-induced antinociception and dopaminergic functionality in the PAG by assessing tyrosine hydroxylase (TH) immunoreactivity. Rats that were subjected to 96 h of PSD received vehicle, morphine (2.5, 5 or 10 mg/kg), L-DOPA (50 or 100 mg/kg) or L-DOPA (50 mg/kg) + morphine (2.5 and 5 mg/kg) and were tested with a 46 degrees C hot plate 1 h after. The paw withdrawal latency responses to the hot plate were decreased in PSD rats and were modified by the highest dose of morphine, L-DOPA and L-DOPA + morphine. Analgesic effects were observed in control groups for all of the morphine doses as well as 100 mg/kg of L-DOPA and L-DOPA (50 mg/kg) + morphine (5 mg/kg). The number of cell bodies that were immunopositive for TH in the PAG was reduced in PSD rats. In conclusion, increased thermal sensitivity was reversed by L-DOPA and could be caused by a reduction TH levels in the PAG. Our data also suggest a relationship between central dopaminergic networks and opiate-induced analgesia in rats. (C) 2011 Elsevier Inc. All rights reserved.
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