期刊
TUMOR BIOLOGY
卷 37, 期 6, 页码 8007-8018出版社
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-015-4656-8
关键词
Esophageal adenocarcinoma; Esophageal squamous cell carcinoma; miR-205; Tumor suppressor; Oncogene
类别
资金
- Czech Ministry of Health [NT/13547-4/2012, NV15-34678A]
- CEITEC Central European Institute of Technology [CZ.1.05/1.1.00/02.0068]
- Project RVO (MOU) [00209805]
- Ministry of Education, Youth and Sports [LM2010004]
Esophageal cancer is a malignant disease with poor prognosis, increasing incidence, and ineffective treatment options. MicroRNAs are post-transcriptional regulators of gene expression involved in many biological processes including carcinogenesis. We determined miR-205 expression levels in tumor/non-tumor tissues of 45 esophageal cancer patients using qPCR and found that decreased level of miR-205 in tumor tissue correlates with poor overall survival in esophageal adenocarcinoma patients. Further, we observed significantly higher levels of miR-205 in tumor tissue of esophageal squamous cell carcinoma. Ectopic overexpression of miR-205 in adenocarcinoma cell line SK-GT-4 led to decreased cell proliferation, cell cycle arrest in G1, and decreased migration ability. Conversely, in squamous cell line KYSE-150, same effects like inhibition of proliferation, migration, and colony-forming potential and cell cycle arrest in G2 were observed after silencing of miR-205. We performed global gene expression profiling and revealed that suppressive functioning of miR-205 in adenocarcinoma could be realized through regulation of epithelial-mesenchymal transition (EMT), whereas oncogenic in squamous cell carcinoma by regulation of metalloproteinase 10. Our results suggest that miR-205 could serve as biomarker in esophageal cancer and acts as a tumor suppressor in esophageal adenocarcinoma and oncogene in esophageal squamous cell carcinoma.
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