Protective effects of curcumin against genotoxicity induced by 131-iodine in human cultured lymphocyte cells

Background: 131-radioiodine has been widely used as an effective radionuclide for treatment of patients with thyroid diseases. The purpose of the present study is to investigate the radioprotective effects of curcumin as a natural product that protects against the genotoxic effects of (131) I in human cultured lymphocytes. Materials and Methods: Whole blood samples from human volunteers were incubated with curcumin at doses of 5, 10, and 50 g/mL. After 1-hour incubation, the lymphocytes were incubated with (131) I (100 Ci/1.5 ml) for 2 hours. The lymphocyte cultures were then mitogenically stimulated to allow for evaluation of the number of micronuclei in cytokinesis-blocked binucleated cells. Results: Incubation of lymphocytes with (131) I at dose 100 Ci/1.5 mL induced genotoxicity shown by increase in micronuclei frequency in human lymphocytes. Curcumin at 5, 10, and 50 g/mL doses significantly reduced the micronuclei frequency. Maximal protective effects and greatest decrease in micronuclei frequency were observed when whole blood was incubated with 50 g/mL dose of curcumin with 52%. Conclusion: This study has important implications for patients undergoing (131) I therapy. Our results indicate a protective role for curcumin against the genetic damage and side effects induced by (131) I administration.