期刊
PHARMACOGNOSY MAGAZINE
卷 8, 期 32, 页码 300-307出版社
MEDKNOW PUBLICATIONS & MEDIA PVT LTD
DOI: 10.4103/0973-1296.103659
关键词
Atractylodes macrocephala; chemical fingerprinting; hierarchical cluster analysis; high-performance liquid chromatography; processed
资金
- Medical Science Research Fund of the Ministry of Health of the People's Republic of China [WKJ2010-2-019]
- National Natural Science Foundation of China [81202918, 81274056, 30873438, 81173546]
- Natural Science Foundation of Jiangsu Province, China [BK2009495]
- International Science and Technology Cooperation Project of Jiangsu Province, China [BZ2011053]
- Open Project of National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine [2011ZYX2-006, 2011ZYX2-001]
- Fund of Zhejiang Modernization of Traditional Chinese Medicine Item [[2008]436]
- Chinese Medicine Research Program of Zhejiang Province, China [2008ZA002]
Background: In China, Atractylodes macrocephala is mainly distributed in Zhejiang province. It is, therefore, desirable to determine a reliable and accurate methodology to differentiate the samples collected from Zhejiang province. Although some studies on the fingerprints of Atractylodes macrocephala using HPLC have been published, none of them compared the processed product of Atractylodes macrocephala from different areas of Zhejiang province. Materials and Methods: Reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with hierarchical cluster analysis was employed in the fingerprint analysis of Atractylodes macrocephala from Zhejiang province, China. The LC assay was performed on a reversed-phase C-18 column with linear gradient elution using water and acetonitrile. The LC data showed considerable variation of chemical constituents among Atractylodes macrocephala populations. Results: 21 and 22 characteristic peaks in the 14 grants of Atractylodes macrocephala and its processed product were determined in samples from different habitats of Zhejiang province, respectively. Their chromatographic patterns were generally consistent although their contents of chemical compositions were greatly different. The results of hierarchical cluster analysis showed that the samples could be divided into four groups; it was able to select excellent resources from the groups. Conclusion: This was the first report of hierarchical cluster analysis of crude and processed Atractylodes macrocephala according to their chemical fingerprints and could be applied to the intrinsic quality control of crude and processed Atractylodes macrocephala. The processing technique of Atractylodes macrocephala through the pilot-scale experiment was first studied and is simple and suitable for prepared yin plan of Atractylodes macrocephala industrial manufacture.
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