期刊
PHARMACEUTICAL RESEARCH
卷 31, 期 4, 页码 946-958出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-013-1214-1
关键词
DMBA induced breast tumor; hepatotoxicity; quercetin; SNEDDS; tamoxifen
资金
- Department of Science & Technology (DST), Government of India, New Delhi
- Council of Scientific and Industrial Research (CSIR), GoI, New Delhi
The present work focuses on the in vivo evaluation of tamoxifen and quercetin combination loaded into solid self-nanoemulsifying drug delivery system (s-Tmx-QT-SNEDDS). Lyophilization was employed to prepare s-Tmx-QT-SNEDDS using Aerosil 200 as carrier. The developed formulation was evaluated for in vitro cell cytotoxicity, in vivo pharmacokinetics, antitumor efficacy and toxicity studies. In vivo pharmacokinetics revealed similar to 8-fold and similar to 4-fold increase in oral bioavailability of tamoxifen and quercetin, respectively as compared to free counterparts. s-Tmx-QT-SNEDDS exhibited significantly higher cell cytotoxicity, as compared to free drug combination revealing similar to 32-fold and similar to 22-fold higher dose reduction index for tamoxifen and quercetin, respectively estimated using median effect dose analysis. s-Tmx-QT-SNEDDS could suppress tumor growth in DMBA induced tumor bearing animals by similar to 80% in contrast to similar to 35% observed with tamoxifen citrate. The significant appreciation in antitumor efficacy was further supported by normalized levels of tumor angiogenesis markers (MMP-2 and MMP-9). Finally, complete obliteration in tamoxifen induced hepatotoxicity was observed upon administration of developed formulation in contrast to that of clinically available tamoxifen citrate when measured as function of hepatotoxicity markers and histopathological changes. In nutshell, co-encapsulation of quercetin with tamoxifen in solid SNEDDS poses great potential in improving the therapeutic efficacy and safety of tamoxifen.
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