期刊
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
卷 460, 期 5, 页码 915-923出版社
SPRINGER
DOI: 10.1007/s00424-010-0871-8
关键词
Endothelium; Mechanical properties; Endothelium-derived relaxing factor (EDRF); Nitric oxide synthase; Aldosterone
类别
资金
- University of Munster
- Deutsche Forschungsgemeinschaft [OB 63/17-1, OB 63/18]
In the vascular endothelium, mechanical cell stiffness (De) and nitric oxide (NO) release are tightly coupled. Soft cells release more NO compared to stiff cells. Currently, however, it is not known whether NO itself is the primary factor that softens the cells or whether NO release is the result of cell softening. To address this question, a hybrid fluorescence/atomic force microscope was used in order to measure changes in De and NO release simultaneously in living vascular endothelial cells. Aldosterone was applied to soften the cells transiently and to trigger NO release. NO synthesis was then either blocked or stimulated and, simultaneously, De was measured. Cell indentation experiments were performed to evaluate De, while NO release was measured either by an intracellular NO-dependent fluorescence indicator (DAF-FM/DA) or by NO-selective electrodes located close to the cell surface. After the application of aldosterone, De decreases, within 10 min, to 80.5 +/- 1.7% of control (100%). DAF-FM fluorescence intensity increases simultaneously to 132.9 +/- 2.2%, which indicates a significant increase in the activity of endothelial NO synthase (eNOS). Inhibition of eNOS (by N (omega)-nitro-l-arginine methyl ester) blocks the NO release, but does not affect the aldosterone-induced changes in De. Application of an eNOS-independent NO donor (NONOate/AM) raises intracellular NO concentration, but, again, does not affect De. Data analysis indicates that a decrease of De by about 10% is sufficient to induce a significant increase of eNOS activity. In conclusion, these nanomechanic properties of endothelial cells in vascular endothelium determine NO release, and not vice versa.
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