期刊
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
卷 457, 期 2, 页码 475-484出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00424-008-0515-4
关键词
TRPC1; Cell migration; Polarity; Calcium signaling
类别
资金
- Human Frontiers Research [RGP 32/2004]
- Excellentiefinanciering [EF/95/010]
- Interuniversity Poles of Attraction Program
Cell migration depends on the generation of structural asymmetry and on different steps: protrusion and adhesion at the front and traction and detachment at the rear part of the cell. The activity of Ca2+ channels coordinate these steps by arranging intracellular Ca2+ signals along the axis of movement. Here, we investigated the role of the putative mechanosensitive canonical transient receptor potential channel 1 (TRPC1) in cell migration. We analyzed its function in transformed renal epithelial (Madin-Darby canine kidney-focus) cells with variation of TRPC1 expression. As shown by time lapse video microscopy, TRPC1 knockdown cells have partially lost their polarity and the ability to persistently migrate into a given direction. This failure is linked to the suppression of a local Ca2+ gradient at the front of migrating TRPC1 knockdown cells, whereas TRPC1 overexpression leads to steeper Ca2+ gradients. We propose that the Ca2+ signaling events regulated by TRPC1 within the lamellipodium determine polarity and directed cell migration.
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