期刊
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
卷 456, 期 1, 页码 227-235出版社
SPRINGER
DOI: 10.1007/s00424-007-0410-4
关键词
caveola; endocytosis; fluorescence; membrane topology; membrane transport
类别
资金
- BBSRC [BB/D020875/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [C20199, BB/D020875/1] Funding Source: Medline
We introduce a novel high resolution scanning surface confocal microscopy technique that enables imaging of endocytic pits in apical membranes of live cells for the first time. The improved topographical resolution of the microscope together with simultaneous fluorescence confocal detection produces pairs of images of cell surfaces sufficient to identify single endocytic pits. Whilst the precise position and size of the pit is detected by the ion conductance microscope, the molecular nature of the pit, e.g. clathrin coated or caveolae, is determined by the corresponding green fluorescent protein fluorescence. Also, for the first time, we showed that flotillin 1 and 2 can be found co-localising with similar to 200-nm indentations in the cell membrane that supports involvement of this protein in endocytosis.
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