期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 104, 期 1, 页码 65-71出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2012.07.002
关键词
Bacillus; Chitinase; Enzyme; Molecular masses; Cutworm; Dietary utilization; Mortality
资金
- Department of Science and Technology (DST), Government of India [SR/FT/LS-161/2010]
An extracellular chitinase was purified from Bacillus subtilis. The lethal concentration (LC50) was determined by using chitinase in first, second, and third instars of Spodoptera litura Fab. Chitinase showed the highest insecticidal activity at 6 mu M concentration within 48 h. The nutritional indices were also significantly affected by the 6 mu M concentration (P < 0.05). Food consumption, efficiency of conversion of ingested and digested food, relative growth rate, and consumption values declined significantly while approximate digestibility was increased. Our study indicates that treatment of host plant leaves with the chitinase can regulate (reduce) larval growth and weight, and enhance the mortality. This may serve as an effective biocide and alternative to Bt toxin. (C) 2012 Elsevier Inc. All rights reserved.
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