期刊
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
卷 95, 期 1, 页码 28-32出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2009.05.001
关键词
Termites trehalase; Inhibition; Validamycin A; Validoxylamine A; Validamycin B; Validoxylamine B
资金
- National High Technology Research and Development Program of China [2006AA10A209]
- National Natural Sciences Foundation of China [20872132]
- Research Program of Science and Technology Department of Zhejiang Province [2008C32026, 2008C02007-3]
Trehalase, with the target to control insects, nematodes and fungi, is of increasing interest and has been investigated extensively in recent years. Validamycin compounds, as competitive trehalase inhibitors and lead compounds with broad applications have attracted substantial attention as well. In this study, the characterizations of termites trehalase were investigated and the inhibitory effects of validamycin compounds on the termites trehalase were studied as well. Results showed that the termites trehalase is presumably belonging to the acid trehalase with optimal pH of 3.3 and optimal temperature of 37 degrees C. It was investigated that the concentrations of validoxylamine A (VAA), validoxylamine B (VBB), validamycin A (VA) and validamycin B (VB) required for 50% inhibition IC50 of termites trehalase were calculated to be 14.73 mg l(-1), 20.80 mg l(-1), 3.17 x 10(3) mg l(-1) and 2.24 x 10(3) mg l(-1), respectively. The inhibition kinetic constant K-i values for the above validamycin compounds were 3.2 x 10(-6) mol l(-1), 1.03 x 10(-5) mol l(-1) 4.02 x 10(-4) mol l(-1) and 2.69 x 10(-4) mol l(-1), respectively. Validoxylamine A appeared to be the most potential termites trehalase inhibitor among the four compounds. (C) 2009 Elsevier Inc. All rights reserved.
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