期刊
PEST MANAGEMENT SCIENCE
卷 70, 期 2, 页码 278-285出版社
WILEY
DOI: 10.1002/ps.3556
关键词
Chenopodium album; psbA gene; photosystem II inhibitor; cross-resistance; PCR-RFLP
资金
- Makhteshim-Agan Group, Israel
BACKGROUNDResistance of Chenopodium album to triazinones and triazines can be caused by two amino acid exchanges, serine-264-glycine (Ser(264)Gly) and alanine-251-valine (Ala(251)Val), in the chloroplast D1 protein. This paper describes the identification of a biotype with a leucine-218-valine (Leu(218)Val) switch found in German sugar beet fields with unsatisfactory weed control. A greenhouse experiment has been performed to compare the resistance profile of the newly identified biotype with biotypes that carry the Ser(264)Gly and Ala(251)Val mutations. RESULTSApplication rate-response curves obtained from the greenhouse experiment showed that the Leu(218)Val exchange induced significant resistance against the triazinones but not against terbuthylazine. The level of resistance against the triazinones was higher in the Ser(264)Gly and Ala(251)Val biotypes compared with the Leu(218)Val biotype. All biotypes tested were more resistant to metribuzin than to metamitron. Following terbuthylazine treatment, Ser(264)Gly displayed a high level of resistance, Ala(251)Val showed moderate resistance. A PCR-RFLP assay for Ser(264)Gly has been extended to include detection of Ala(251)Val and Leu(218)Val mutations. CONCLUSIONThe D1 Leu(218)Val substitution in C. album confers significant resistance to triazinones. This suggests that Leu(218)Val is involved in the binding of triazinones. First establishment of the resistance profiles of the three psbA mutations suggests that these mutations have been independently selected. (c) 2013 Society of Chemical Industry
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