期刊
PEST MANAGEMENT SCIENCE
卷 70, 期 7, 页码 1048-1058出版社
WILEY
DOI: 10.1002/ps.3645
关键词
Cyp6a2; DDT resistance; frameshift mutation; long terminal repeat; Nrf2/Maf binding site; overexpression
资金
- National Basic Research Program of China (973 program) [2012CB114103]
- National Natural Science Foundation of China [30870342]
BACKGROUND: Increased insecticide detoxification mediated by cytochrome P450s is a common mechanism of insecticide resistance. Although Cyp6a2 has been observed to be overexpressed in many 4,4'-dichlorodiphenyltrichloroethane (DDT)-resistant strains of Drosophila melanogaster, how Cyp6a2 is regulated and whether its overproduction confers DDT resistance remain elusive. RESULTS: Molecular analysis identified five Cyp6a2 alleles (Cyp6a2(Canton-S-1), Cyp6a2(Canton-S-2), Cyp6a2(91-C), Cyp6a2(91-R) and Cyp6a2(Wisconsin-WD)) from four D. melanogaster strains, notably differing in the presence or absence of an intact Nrf2/Maf (a transcription factor) binding site in the 5'-promoter core region, a 'G1410' frameshift deletion mutation in the heme-binding region and a long terminal repeat (LTR) of transposable element 17.6 in the 3'-untranslated region (UTR). Linkage analysis confirmed that DDT resistance was genetically linked to a Nrf2/Maf-binding-site-containing, LTR-lacking functional allele of Cyp6a2 (Cyp6a2(91-R)). The qRT-PCR results showed that overexpression of functional Cyp6a2 was consistently associated with DDT resistance. Luciferase reporter gene assays revealed that an intact Nrf2/Maf binding site in the 5'-promoter core region enhanced the constitutive transcription of Cyp6a2. CONCLUSION: The results suggest that the Nrf2/Maf binding-site-containing functional Cyp6a2 allele is associated with DDT resistance in the D. melanogaster strains under study. (C) 2013 Society of Chemical Industry
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