期刊
PEST MANAGEMENT SCIENCE
卷 68, 期 9, 页码 1241-1247出版社
WILEY
DOI: 10.1002/ps.3285
关键词
acetyl-CoA carboxylase; herbicide resistance; Alopecurus japonicus; haloxyfop-R-methyl; amino acid substitution
资金
- National Natural Science Foundation of China [30971928]
- Specialised Research Fund, Doctoral Programme of Higher Education of China [20090097120046]
BACKGROUND: Haloxyfop-R-methyl is a widely used herbicide to control Poaceae weeds. Alopecurus japonicus, a widespread annual grass, can no longer be controlled by haloxyfop-R-methyl after continuous use of this herbicide for several years. RESULTS: Dose-response experiments have established that the Js-R biotype of A. japonicas has evolved resistance to aryloxyphenoxypropionates (APPs). Target-site enzyme sensitivity experiments have established that the haloxyfop (free acid) rate causing 50% inhibition of acetyl-CoA carboxylase (ACCase) activity (I50) for the resistant (Js-R) biotype is 11 times higher than that for the susceptible (Js-S) biotype. In many cases, resistance to ACCase-inhibiting herbicides is due to a resistant ACCase enzyme. Full-length DNA and mRNA sequences of the plastidic ACCase gene were amplified. Eight single-nucleotide differences were detected in this region. Four of the nucleotide changes were silent mutations. However, the other four nucleotide mutations caused four amino acid substitutions, replacing Arg-1734 with Gly, Met-1738 with Leu, Thr-1739 with Ser and Ile-2041 with Asn in the R biotype respectively; the substitution at position 2041 had been reported, while the other three had not. CONCLUSION: The ACCase in the Js-R biotype was less susceptible to haloxyfop-R-methyl than that in the Js-S biotype. Moreover, the amino acid substitution of Ile-2041 with Asn might confer resistance to haloxyfop-R-methyl in A. japonicas. Copyright (c) 2012 Society of Chemical Industry
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