期刊
PEST MANAGEMENT SCIENCE
卷 66, 期 9, 页码 949-955出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.1965
关键词
medfly; Ceratatis capitata; Sesamia nonagrioides; Culex pipiens; Bacillus thuringiensis; proteases; solubilisation; delta-endotoxins
资金
- Spanish Ministry of Education and Science (MEC) [AGL2006-13346-CO2-O1/AGR]
- Conselleria de Agricultura Pesca y Alimentacion, Generalitat Valenciana [GV 2009-5926]
- Instituto Nacional de Investigaciones Agrarias (INIA)
BACKGROUND: Ceratitis capitata (Wiedemann) is one of the world's most destructive fruit pests. The aim of this study was to ascertain insecticidal activity of Bacillus thuringiensis (Berliner) delta-endotoxins to C. capitata. RESULTS: Among 42 selected Bacillus strains, only B. thuringiensis serovar israelensis (Bti) solubilised protoxins showed biological activity against C. capitata neonate larvae, whereas Bti spore and crystal mixture was inactive. Insecticidal activity of Bti protoxins was significantly enhanced by incubation with Culex pipiens L. gut extracts. Overdigestion of Bti protoxins with Sesamia nonagrioides (Lefebvre) gut extracts suppressed biological activity against C. capitata, and this correlated with degradation of Cyt toxins. Cyt1Aa solubilised protoxin showed the highest toxicity, LC50 after 7 days of 4.93 mu g cm(-2), while proteolytical processing of Cyt1Aa protoxins by larval gut extracts did not enhance insecticidal activity. CONCLUSION: The present study provides evidence for the first time of the insecticidal activity of a B. thuringiensis strain against C. capitata and identifies a single delta-endotoxin with potential for controlling this pest. (C) 2010 Society of Chemical Industry
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