Molecular characterization of virulence factors in Aeromonas hydrophila obtained from fish

Oliveira S. T. L., Veneroni-Gouveia G. & Costa M. M. 2012. Molecular characterization of virulence factors in Aeromonas hydrophila obtained from fish. Pesquisa Veterinaria Brasileira 32(8): 701-706. Laboratorio de Microbiologia e Imunologia Animal, Universidade Federal do Vale do Sao Francisco, Campus Ciencias Agrarias, Colegiado Academico de Zootecnia, Rodovia BR 407 Km 12, Lote 543, Projeto de Irrigacao Nilo Coelho s/n, C1, Petrolina, PE 56300-000, Brazil. E-mail: mateus. costa@univasf.edu.br Multiple factors can be involved in the virulence processes of Aeromonas hydrophila. The objective of the present paper was to verify the presence of aerolysin, hidrolipase, elastase and lipase virulence genes through the polymerase chain reaction (PCR) in A. hydrophila isolates obtained from fish of the Sao Francisco River Valley, and to evaluate virulence according to the presence of these genes in Nile tilapia fingerlings. One hundred and fourteen isolates from the bacteria were used. DNA was heat extracted and PCR undertaken using specific primers described in the literature. For in vivo tests Nile tilapia fingerlings were used. From the PCR tests, negative isolates for all genes tested were selected, positive isolates for two genes (aerolysin and elastase) and positive for the four genes tested. These were inoculated at a concentration of 108 UFC/ml into the tilapias, considered as treatments; another group of animals was used as control (with inoculation of saline solution). In all, 12 distinct standards regarding the presence of virulence factors in isolates from A. hydrophila, were observed. Of the 114 isolates analyzed, 100 (87.72%) presented at least one of the virulence factors under study. The virulence factors were widely distributed among the A. hydrophila isolates. Aerolysin was the most frequent virulence factor present in the isolates analyzed. A. hydrophila led to the mortality of the Nile tilapia fingerlings, regardless of the absence or quantity of virulence genes tested.