期刊
PESQUISA VETERINARIA BRASILEIRA
卷 29, 期 11, 页码 874-880出版社
REVISTA PESQUISA VETERINARIA BRASILEIRA
DOI: 10.1590/S0100-736X2009001100002
关键词
Oreochromis niloticus; vaccine; hematology; immunology
资金
- CNPq [CNPq301072/2007-8, CNPq 472968/2007-6]
- Microbiology Department of the Marine Shrimp Laboratory/LCM-UFSC
The efficacy of a polyvalent bacterin vaccine against Aeromonas hydrophila, Pseudomonas aeroginosa and Enterococcus durans administered by different routes in Nile tilapia was assessed by analyzing hematological and immunological parameters 7 and 21 days after vaccination. Treatments consisted of: non-vaccinated tilapia; tilapia vaccinated by intraperitoneal injection with 2x10(8) formalin-inactivated bacteria.mL(-1); tilapia vaccinated orally with 2x10(7) formalin-inactivated bacteria.g(-1), feed for 5 days; tilapia vaccinated by immersion bath in 2x10(7) formalin-inactivated bacteria.mL(-1), for 20 minutes. Vaccinated fish groups presented higher hematocrit, number of erythrocytes and leukocytes than the non-vaccinated group. Serum agglutination titer of intraperitoneally vaccinated fish was higher on both evaluation periods for the three bacteria strains. Only on day 21 post-vaccination fish from the oral and immersion vaccination groups presented higher serum agglutination titer than the non-vaccinated fish for A. hidrophyla and E durans. Serum antimicrobial activity in vaccinated fish was higher for P. aeroginosa and E coli than in non-vaccinated fish on both evaluation periods. The different vaccine administration routes stimulated hematological and immunological responses in Nile tilapia 21 days post-vaccination, but intraperitoneal vaccination presented higher total number of leukocytes, lymphocytes and serum agglutination titer.
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