期刊
PERSOONIA
卷 21, 期 -, 页码 57-69出版社
RIJKSHERBARIUM
DOI: 10.3767/003158508X361334
关键词
aleurioconidium; chlamydospore; cytochrome oxidase subunit I (Cox1); internal transcribed spacer (ITS); Leotiomycetes
类别
资金
- Canadian Barcode of Life Network and Genome Canada
- NSERC
Three new species of Leohumicola (anamorphic Leotiomycetes) are described using morphological characters and phylogenetic analyses of DNA barcodes. Leohumicola levissima and L. atra were isolated from soils collected after forest fires in Crater Lake National Park, United States. Leohumicola incrustata was isolated from burned fynbos from the Cape of Good Hope Nature Reserve, South Africa. The three species exhibit characteristic Leohumicola morphology but are morphologically distinct based on condial characters. Two DNA barcode regions, the Internal Transcribed Spacer (ITS) nuclear rDNA region and the cytochrome oxidase subunit I (Cox 1) mitochondrial gene, were sequenced. Single-gene parsimony, dual-gene parsimony and dual-gene Bayesian inference phylogenetic analyses support L. levissima, L. atra, L. incrustata as distinct phylogenetic species. Both ITS and Cox1 barcodes are effective for the molecular identification of Leohomicola species.
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