期刊
PEPTIDES
卷 53, 期 -, 页码 307-314出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2013.12.014
关键词
Insects; Hemiptera; Acyrthosiphon pisum; Aphids; RNAi; dsRNA degradation
资金
- Special Research Fund of Ghent University
- Fund for Scientific Research (FWO-Vlaanderen, Brussels)
Over the past decade, RNA interference (RNAi), the sequence-specific suppression of gene expression, has proven very promising for molecular research in many species, including model insects as Tribolium castaneum and Apis mellifera. It showed its usefulness to analyze gene function and its potential to managepest populations and reduce disease pathogens. However, in several insects, the efficiency of RNAi is lowor very variable at best. One of the factors that could influence RNAi efficiency in insects is degradation of dsRNA after administration to the insect. In this paper, we report on the importance of dsRNA break-down in the pea aphid (Acyrthosiphon pisum) associated with the absence of an RNAi response upon oral feeding and injection with dsRNA targeting different genes such as the ecdysone hormone receptor and ultraspiracle. In essence, we discovered that both the salivary secretions of aphids and the hemolymph were able to degrade the dsRNA. In parallel, introduction of dsRNA in the aphid body was not able to provoke a response in the expression of the siRNA core machinery genes. (c) 2013 Elsevier Inc. All rights reserved.
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