期刊
PEPTIDES
卷 41, 期 -, 页码 17-20出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2013.01.009
关键词
Conidae; Conopeptide; Conotoxin; Conus delessertii; Hydroxy-lysine; Peptide de13b
资金
- Consejo Nacional de Ciencia y Tecnologia, Mexico (CONACYT) [79929]
- Direccion General de Asuntos del Personal Academico, Universidad Nacional Autonoma de Mexico [IN-204403]
- Australian Research Council [DC010195]
Peptide de13a was previously purified from the venom of the worm-hunting cone snail Conus delessertii from the Yucatan Channel, Mexico. This peptide has eight cysteine (Cys) residues in the unique arrangement C-C-C-CC-C-C-C, which defines the cysteine framework XIII (- represents one or more non-Cys residues). Remarkably, delta-hydroxy-lysine residues have been found only in conotoxin de13a, which also contains an unusually high proportion of hydroxylated amino acid residues. Here, we report the cDNA cloning of the complete precursor De13.1 of a related peptide, de13b, which has the same Cys framework and inter-Cys spacings as peptide de13a, and shares high protein/nucleic acid sequence identity (87%/90%) with de13a, suggesting that both peptides belong to the same conotoxin gene superfamily. Analysis of the signal peptide of precursor De13.1 reveals that this precursor belongs to a novel conotoxin gene superfamily that we chose to name gene superfamily G. Thus far superfamily G only includes two peptides, each of which contains the same, distinctive Cys framework and a high proportion of amino acid residues with hydroxylated side chains. (c) 2013 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据