期刊
PEPTIDES
卷 36, 期 1, 页码 114-120出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2012.03.002
关键词
Mastoparan-AF; Antimicrobial resistance gene; Antimicrobial activity; Antibiotic; E. coli; Synergism
资金
- National Science Council, Taiwan, ROC [NSC98-2313-B-005-024-MY3]
The in vitro activity of mastoparan-AF, an amphipathic antimicrobial peptide isolated from the hornet venom of Vespa affinis, alone and in combination with various clinically used antibiotics, was investigated against 21 Escherichia coli isolates/strains. Most E. coli isolates tested were detected containing multiple-antimicrobial resistance genes. Antimicrobial activity of mastoparan-AF was measured by MIC, MBC, time-kill kinetic assay and chequerboard titration method. Mastoparan-AF exhibited potent antimicrobial activity against most multiple-antibiotic-resistant E. coli isolates at the concentrations ranging from 4 to 16 mu g/ml. Combination studies showed that mastoparan-AF acts synergistically with certain antibiotics, i.e., cephalothin or gentamicin, against some multiple-antibiotic-resistant E. coli isolates. In conclusion, mastoparan-AF alone or in combination with other antibiotics could be promising as alternatives for combating multiple-antibiotic-resistant E. coli in future clinical applications. (C) 2012 Elsevier Inc. All rights reserved.
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