期刊
PEPTIDES
卷 36, 期 1, 页码 9-16出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2012.03.019
关键词
LVV-hemorphin 7; Angiotensin IV; Insulin-regulated aminopeptidase (IRAP); Hyperalgesia; Carrageenan; Spinal cord
资金
- National Science Council, Taiwan, R.O.C [NSC 96-2320-B-016-020-MY3]
- Chi Mei Medical Center, Tainan, Taiwan, R.O.C [CMFHR9639]
Hemorphins, a family of atypical endogenous opioid peptides, are produced by the cleavage of hemoglobin beta-chain. Hemorphins were proved to bind to the mu-opioid receptors (agonist) and angiotensin IV receptors (insulin-regulated aminopeptidase; IRAP) (inhibitor). Among the hemorphins, LVV-hemorphin-7 (LVV-H7) was found to be abundant and with a longer half life in the CNS. Using intrathecal and intracerebroventricular injections, LVV-H7 and angiotensin IV were given to the rats, which were then subjected to the plantar test and the tail-flick test. Our results showed that LVV-H7 attenuated carrageenan-induced hyperalgesia at the spinal level, which could not be reversed by the co-administration of naloxone. At the supraspinal level, LVV-H7 also produced a significant anti-hyperalgesia effect but with a lower extent. Angiotensin IV showed a similar anti-hyperalgesia effect at the spinal level, but had no effect at the supraspinal level. In the tail-flick test and paw edema test, both peptides showed no effect. These results suggest that LVV-H7 mainly exert the anti-hyperalgesia effect at the spinal level, possibly through IRAP but not mu-opioid receptors. In addition, we observed the expression of IRAP in the CNS of animals with/without carrageenan-induced hyperalgesia. Our results showed a significant expression of IRAP in the spinal cord of rats. However, there was no significant quantitative change of IRAP after the development of hyperalgesia. The serum level of LVV-H7 was also found to be with no change caused by hyperalgesia. These results indicated that the endogenous LVV-H7 and IRAP may not regulate the severity of hyperalgesia through a quantitative change. (C) 2012 Elsevier Inc. All rights reserved.
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