期刊
PEPTIDES
卷 34, 期 1, 页码 19-25出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2011.10.029
关键词
Tetrodotoxin-sensitive voltage-gated sodium channel; Dorsal root ganglion; Dorsal unpaired median; Hippocampal neuron
资金
- 973 Program [2010CB529801]
- NSFC [31000477]
- SRFDP [20104306120005]
- Chinese Ministry of Education [208096]
- Hunan Provincial Education Department [10C0961]
- Fok Ying Tung Education Foundation [111023]
Huwentoxin-I (HWTX-I) is a 33-residue peptide isolated from the venom of Ornithoctonus huwena and could inhibit TTX-sensitive voltage-gated sodium channels and N-type calcium channels in mammalian dorsal root ganglion (DRG) neurons. However, the effects of HWTX-I on mammalian central neuronal and insect sodium channel subtypes remain unknown. In this study, we found that HWTX-I potently inhibited sodium channels in rat hippocampal and cockroach dorsal unpaired median (DUM) neurons with the IC50 values of 66.1 +/- 5.2 and 4.80 +/- 0.58 nM, respectively. Taken together with our previous work on DRG neurons (IC50 approximate to 55 nM), the order of sodium channel sensitivity to HWTX-I inhibition was insect central DUM >> mammalian peripheral > mammalian central neurons. HWTX-I exhibited no effect on the steady-state activation and inactivation of sodium channels in rat hippocampal and cockroach DUM neurons. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据