期刊
PEPTIDES
卷 30, 期 8, 页码 1421-1427出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2009.05.016
关键词
Antimicrobial peptide; Buforin 2; Analog; Membrane action; Mechanism
资金
- National Natural Science Foundation of China (NSFC) [30871805]
- 111 project [B07029]
Previously, the antimicrobial peptides BF2-A and BF2-B, two analogs of Buforin 2 that was hypothesized to kill bacteria by entering cells and binding nucleic acids, had been designed based on the structure-activity analysis of Buforin 2. In the present study, BF2-A and BF2-B were chemically synthesized and their activities and lipopolysaccharide affinity were assayed. To elucidate the mechanism of action with cytoplasmic membranes, we subsequently examined the membrane permeability of both peptides in detail. Both peptides showed stronger antimicrobial activities against a broad spectrum of microorganisms than their parent peptide. Interestingly, BF2-A did not cause significant membrane permeabilization for influx of ONPG into cells, and hardly caused the leakage of intracellular macromolecules, probably BF2-A slightly disturbed cell membrane causing the K+ leakage during peptide crossing phospholipids bilayer. Electron micrographs indicated that the cell membrane treated by BF2-A was still intact within 20 min. On the contrary, BF2-B obviously increased the outer and inner membrane permeability, even induced the slight leakage of macromolecules in the cytoplasm. The leakage of cytoplasmic contents was also demonstrated by the electron micrographs. The increase of membrane permeabilization explained why BF2-B displayed better antimicrobial activity and rapid killing kinetics than BF2-A. (C) 2009 Elsevier Inc. All rights reserved.
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