期刊
PEPTIDES
卷 30, 期 7, 页码 1336-1341出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2009.03.013
关键词
RNAi; Ghrelin; Paraventricular nucleus; Hypothalamus; GSHR1a
资金
- NIDDK NIH HHS [R01 DK059836-01, R01 DK059836] Funding Source: Medline
Central and peripheral injections of fghrelin potently stimulates food intake via its receptor, GHSR1a expressed in the brain. In this study, we explored the role of GHSR1a in the paraventricular nucleus of the hypothalamus (PVN) by reducing their gene expression using the RNA interference (RNAi). pSUPER plasmids inserted with sh (short hairpin)-GHSR1a were injected into the PVN to reduce its expression. The transfected rats were monitored daily for their food intake and body weight throughout the experimental period lasting 8 days. We found that knockdown of GHSR1a did not affect daily food intake but significantly reduced body weight and blood ghrelin levels. This suggests that the central ghrelin system could selectively regulate body weight without affecting energy intake. (C) 2009 Elsevier Inc. All rights reserved.
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