期刊
PEPTIDES
卷 29, 期 10, 页码 1679-1684出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2008.06.001
关键词
Ornithoctonus huwena; Spider; Intron; Genomic organization; Toxin-like peptide
资金
- National Natural Science Foundation of China [30430170, 30870640, 30700127]
The bird spider Ornithoctonus huwena is one of the most venomous spiders in China. Its venom is a mixture of various compounds with diverse bioactivities. Ninety proteins and 47 peptides have been identified, and 67 cDNA sequences encoding different toxin precursors have been cloned. However, the genomic DNA of them is seldom reported. To characterize the genomic DNA structure of huwentoxins, the genomic DNA encoding toxins of three superfamilies were cloned by using sequence specific or partially degenerate primers based on their cDNA sequences. An unexpected finding was that the intron was lacking in the genomic sequences of three superfamilies. The genomic DNA information has predictive value for better understanding the relationship of spider toxin evolution. In addition, we have cloned and analyzed 19 novel genes encoding toxin-like precursors by using the genomic DNA of the spider O. huwena. (C) 2008 Published by Elsevier Inc.
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