期刊
TRENDS IN MICROBIOLOGY
卷 23, 期 8, 页码 498-508出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.tim.2015.04.008
关键词
solvent tolerance; bacterial host engineering; biofuel production; efflux pump; transporters
资金
- US Department of Energy (DOE) [DE-AC02-05CH11231]
During microbial production of solvent-like compounds, such as advanced biofuels and bulk chemicals, accumulation of the final product can negatively impact the cultivation of the host microbe and limit the production levels. Consequently, improving solvent tolerance is becoming an essential aspect of engineering microbial production strains. Mechanisms ranging from chaperones to transcriptional factors have been used to obtain solvent-tolerant strains. However, alleviating growth inhibition does not invariably result in increased production. Transporters specifically have emerged as a powerful category of proteins that bestow tolerance and often improve production but are difficult targets for cellular expression. Here we review strain engineering, primarily as it pertains to bacterial solvent tolerance, and the benefits and challenges associated with the expression of membrane-localized transporters in improving solvent tolerance and production.
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