期刊
PEDIATRIC RESEARCH
卷 71, 期 6, 页码 653-660出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/pr.2012.23
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- Regione Friuli Venezia Giulia (Fondo Regionale FVG) [LR26/2005]
- Telethon [GGP10051]
- Italian Ministry of Foreign Affairs, Rome, Italy
- Czech Ministry of Education [2B06155]
- Research Granting Agency of the Czech Republic [CZ:GA CR:P206/11/0836]
INTRODUCTION: Few data exist on regional brain bilirubin content in the neonatal period when acute bilirubin-induced neurologic damage (BIND) may occur, and no information is available on regional brain expression of cytochrome P450 monooxygenases (Cyps) that oxidize bilirubin. METHODS: Bilirubin content was analyzed by high-performance liquid chromatography and Cyp1a1, 1a2, and 2a3 mRNA expression was analyzed by quantitative PCR (qPCR) in cortex (Cx), cerebellum (CII), superior colliculi (SC), and inferior colliculi (IC) of 17-d-old hyperbilirubinemic (jj) Gunn rat pups before and after administration of sulphadimethoxine to acutely displace bilirubin from plasma albumin. RESULTS:There was no difference in bilirubin content among brain regions in untreated rats. After intraperitoneal sulphadimethoxine, bilirubin content peaked at fourfold in Cx and SC at 1h; but at 11- to 13-fold in CII and IC at 24h; returning to control levels at 72h. The Cyp mRNA peaked at 30-70 times control at 1 h in Cx and SC, but at 3-9 times control at 24 h in CII and IC. DISCUSSION: The close relationship in distinct brain regions between the extent of bilirubin accumulation and induction of mRNA of Cyps suggests Cyps may have a role in protecting selected brain areas from bilirubin neurotoxicity.
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