期刊
TRENDS IN BIOTECHNOLOGY
卷 33, 期 3, 页码 145-147出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2014.12.003
关键词
food/feed safety; gene silencing; genetically modified organism (GMO); nontarget organism (NTO); off-target gene; plant breeding
RNAi offers opportunities to generate new traits in genetically modified (GM) plants. Instead of expressing novel proteins, RNAi-based GM plants reduce target gene expression. Silencing of off-target genes may trigger unintended effects, and identifying these genes would facilitate risk assessment. However, using bioinformatics alone is not reliable, due to the lack of genomic data and insufficient knowledge of mechanisms governing mRNA small (s)RNA interactions.
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