期刊
TRENDS IN BIOTECHNOLOGY
卷 33, 期 9, 页码 489-491出版社
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2015.04.002
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资金
- Ministry of Science, ICT & Future Planning, Republic of Korea [IBS-R021-D1-2015-A00] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells.
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