期刊
PATHOLOGY RESEARCH AND PRACTICE
卷 206, 期 3, 页码 163-167出版社
ELSEVIER GMBH
DOI: 10.1016/j.prp.2009.10.007
关键词
Loss of heterozygosity; Tumor suppressor genes; Testicular germ cell tumors
类别
资金
- Ministry of Science and Technology, Republic of Croatia [058-0582261-2246]
Human testicular germ cell tumors (TGCTs) are histologically heterogenous neoplasms with a variable malignant potential. Two main groups of germ cell tumors occur in men: seminomas and nonseminomas. In the present study, a set of four tumor suppressor genes was investigated in testicular cancers. CDH1, APC, p53, and nm23-H1 genes were tested for loss of heterozygosity (LOH). Thirty-eight testicular germ cell tumors (17 seminomas and 21 nonseminomas) were analyzed by PCR using restriction fragment length polymorphism or the dinucleotide/tetranucleotide repeat polymorphism method. An allelic loss of p53 at exon 4 was detected in five nonseminomas, whereas LOH of p53 at intron 6 occurred in one of the seminoma and two of the nonseminoma samples. Allelic losses of the APC gene were present in three seminomas and one nonseminoma, whereas one seminoma and three nonseminomas showed LOH of CDH1. The analysis of allelic losses showed no common structural genetic alterations in tumor tissues, although a different pattern of LOH was observed between the two main histological groups of TGCTs. (c) 2009 Elsevier GmbH. All rights reserved.
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