Immunolocalization of β- and δ-giardin within the ventral disk in trophozoites of Giardia duodenalis using multiplex laser scanning confocal microscopy

Immunolocalization of beta- and delta-giardin in Giardia duodenalis trophozoites revealed that both giardins are strictly associated with the ventral disk (VD). Optical sectioning of the immunolabeled VD, together with quantitative colocalization of delta- and beta-giardin immunoreactivity, demonstrated that delta-giardin is primarily localized to the ventral side, and beta-giardin is localized to the dorsal side of the VD.