期刊
PARASITOLOGY RESEARCH
卷 109, 期 5, 页码 1361-1365出版社
SPRINGER
DOI: 10.1007/s00436-011-2381-9
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资金
- National Natural Science Foundation of China [30571394]
- National Natural Science Foundation of Hebei province [C2007000523]
Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.
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