期刊
PARASITOLOGY
卷 138, 期 14, 页码 1935-1944出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182011001454
关键词
Corridor disease; diagnostic assay; real-time hybridization; Syncerus caffer; Theileria parva
类别
资金
- OVI (Department of Agriculture, Forestry and Fisheries) [15/08/1P01, OV21/03/C148]
Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria parva. This is a nationally controlled disease in South Africa and strict control measures apply for the movement of buffalo, which includes mandatory testing for the presence of T. parva and other controlled diseases. Accurate diagnosis of the T. parva carrier state in buffalo using the official real-time hybridization PCR assay (Sibeko et al. 2008), has been shown to be affected by concurrent infection with T. sp. (buffalo)-like parasites. We describe the Hybrid II assay, a real-time hybridization PCR method, which compares well with the official hybridization assay in terms of specificity and sensitivity. It is, however, not influenced by mixed infections of T. sp. (buffalo)-like parasites and is as such a significant improvement on the current hybridization assay.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据