期刊
PARASITOLOGY
卷 136, 期 10, 页码 1201-1207出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182009990448
关键词
Trypanosoma cruzi; arginine kinase; phosphotransferase; phosphagen; Chagas' disease
类别
资金
- Consejo Nacional de Investigaciones Cientificas y, Tecnicas (CONICET) [PIP 5492]
- Agencia Nacional de Promocion Cientifica v Tecnologica (FONCyT) [PICT 33431]
- FONCYT-PICT [REDES 2003-00300]
Phosphoarginine is a cell energy buffer molecule synthesized by the enzyme arginine kinase. In Trypanosoma cruzi., the aetiological agent of Chagas' disease, 2 different isoforms were identified by data mining, but only 1 was expressed during the parasite life cycle. The digitonin extraction pattern of arginine kinase differed from those obtained for reservosomes, glycosomes and mitochondrial markers, and similar to the cytosolic marker. Immunofluorescence analysis revealed that although arginine kinase is localized mainly in unknown punctuated structures and also in the cytosol, it did not co-localize with any of the subcelular markers. This punctuated pattern has previously been observed in many cytosolic proteins of trypanosomatids. The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their Physiological role in protozoan parasites.
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