期刊
PARASITES & VECTORS
卷 7, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/1756-3305-7-150
关键词
Liver fluke; Fasciola spp; Mitochondrial genome; Phylogenetic analysis
资金
- International Science & Technology Cooperation Program of China [2013DFA31840]
- Special Fund for Agro-scientific Research in the Public Interest [201303037]
- Science Fund for Creative Research Groups of Gansu Province [1210RJIA006]
- Australian Research Council (ARC)
- National Health and Medical Research Council (NHMRC)
- Melbourne Water Corporation (MWC)
- Alexander von Humboldt Foundation
- Victorian Life Sciences Computation Initiative (VLSCI) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government [VR0007]
- NHMRC Early Career Research Fellow
Background: Fascioliasis is an important and neglected disease of humans and other mammals, caused by trematodes of the genus Fasciola. Fasciola hepatica and F. gigantica are valid species that infect humans and animals, but the specific status of Fasciola sp. (`intermediate form') is unclear. Methods: Single specimens inferred to represent Fasciola sp. (`intermediate form'; Heilongjiang) and F. gigantica (Guangxi) from China were genetically identified and characterized using PCR-based sequencing of the first and second internal transcribed spacer regions of nuclear ribosomal DNA. The complete mitochondrial (mt) genomes of these representative specimens were then sequenced. The relationships of these specimens with selected members of the Trematoda were assessed by phylogenetic analysis of concatenated amino acid sequence datasets by Bayesian inference (BI). Results: The complete mt genomes of representatives of Fasciola sp. and F. gigantica were 14,453 bp and 14,478 bp in size, respectively. Both mt genomes contain 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes, but lack an atp8 gene. All protein-coding genes are transcribed in the same direction, and the gene order in both mt genomes is the same as that published for F. hepatica. Phylogenetic analysis of the concatenated amino acid sequence data for all 12 protein-coding genes showed that the specimen of Fasciola sp. was more closely related to F. gigantica than to F. hepatica. Conclusions: The mt genomes characterized here provide a rich source of markers, which can be used in combination with nuclear markers and imaging techniques, for future comparative studies of the biology of Fasciola sp. from China and other countries.
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