Changes in the activity of adult stages of Dermacentor reticulatus (Ixodida: Amblyommidae) induced by weather factors in eastern Poland

Background: The host-seeking activity in Dermacentor reticulatus ticks undergoes rhythmical changes correlated with environmental conditions. Therefore, this study is focused on investigating the activity of adult stages of the species during weather changes occurring in winter months in eastern Poland, i.e. a period of tick diapause. Methods: D. reticulatus ticks were collected in a meadow ecosystem near Lublin (eastern Poland, 51 degrees 36'N, 22 degrees 58'E) between the third decade of November 2011 and the third decade of January 2012. During each collection, temperature and humidity were measured at the soil surface and at a height of 25 cm and the differences (delta) in the parameters between the two measurement points were calculated. Results: During one hour of our observation, from 0 to 42 specimens were collected, with the greatest numbers (25-40 specimens) between late November and mid-December. The activity of adult D. reticulatus (females and males in total) depended on soil and air temperature (r = -0.6986, p < 0.05). Soil and air humidity did not exert an impact on the questing behaviour of adult stages. In turn, the greater the moisture delta between these two measurement points was, the greater the activity of the adult tick stages was observed. Our investigations have demonstrated differences in the questing behaviour between D. reticulatus females and males correlated with environmental conditions. The temperatures of soil and air increased the migratory activity in females but did not affect migration of male ticks. In turn, the deltas of temperatures (r = -0.6986, p < 0.05) and humidity (r = 0.6829, p < 0.05) did not have a statistically significant effect on stimulation of female activity but they induced significant changes on the behaviour of males, for which we found a highly negative correlation between the number of active specimens and the temperature delta (r = -0.7276, p < 0.05) and a highly positive correlation with the humidity delta (r = 0.8199, p < 0.01). Conclusions: Weather changes can be accompanied by activity of adult D. reticulatus even in their winter diapause period. Therefore, there is an increased threat to human and animal health posed by tick-borne diseases.