期刊
PANCREAS
卷 40, 期 6, 页码 905-910出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MPA.0b013e3182207a58
关键词
cell line; small cell carcinoma; pancreas; neuroendocrine neoplasm; molecular genetics
资金
- National Institutes of Health [CA106610, CA62924]
- Uehara Memorial Foundation
- George Rubis Endowment for Pancreatic Cancer Research
- Michael Rolfe Pancreatic Cancer Foundation
- Joseph C. Monastra Foundation
- Alfredo Scatena Memorial
- Patty Boshell Pancreas Cancer Foundation
Objectives: Small cell carcinoma (SCC) of the pancreas is a rare malignancy with a poor prognosis. We established and characterized a primary human pancreatic SCC cell line, designated A99. Methods: Cancer tissue was obtained from the liver metastasis of an SCC of the pancreas and xenografted into nude mice. The first-pass xenograft was then used to establish a cultured cell line called A99. Cellular morphology, immunohistochemical properties, tumorigenic potential, and genetic alterations of this new line were characterized. Results: A99 cells grew consistently in culture, formed colonies in soft agar, and grew as subcutaneous xenografts when inoculated into nude mice. A99 cells were positive for pancytokeratin, synaptophysin, chromogranin A, neuron-specific enolase, CD57 (Leu7), CD56, protein gene product 9.5, thyroid transcription factor 1, Smad4, p53, and p16, but not for CD99, PDX-1, or retinoblastoma protein. Sequencing analysis revealed homozygous point mutations of KRAS and TP53. Cytogenetic analysis revealed complex chromosomal rearrangements including marker chromosomes. Conclusions: A99 is the first cell line reported to be derived from a primary SCC of the pancreas. The establishment of this cell line may serve as a useful model system for studying the cell biology of this rare cancer or for evaluating novel targeted agents in preclinical models.
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