期刊
ARCHIVES OF ORAL BIOLOGY
卷 60, 期 6, 页码 948-958出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2015.03.005
关键词
Inflammasome; RT-PCR; Immunohistochemistry; NLRP3; NLRP1; AIM2
资金
- National Natural Science Foundation of China [81100754]
Objective: Detecting the distribution and intensity of NLRP3, NLRP1 and AIM2 expression in different types of periodontitis gingival tissues. Materials and methods: A total of 65 gingival tissues were collected from clinic and been divided into three groups: patients with chronic periodontitis (CP), patients with generalized aggressive periodontitis (G-AgP) and healthy control subjects. Real-time polymerase chain reaction (RT-PCR) was performed to detect mRNA expression of NLRP3, NLRP1 and AIM2 in full-thickness tissue. In the meanwhile, immunohistochemistry was used to detect distribution of NLRP3, NLRP1 and AIM2 in the periodontal epithelium and in the connective tissue cells. Results: The overall intensity of NLRP3 expression was significantly higher in CP or G-AgP than healthy tissue. A more significant difference was observed in the periodontal epithelium layer. NLRP1 was barely expressed in the healthy and periodontitis gingival tissues, whereas AIM2 was expressed at a higher level in the chronic periodontitis group than others. Conclusion: The NLRP3, NLRP1 and AIM2 proteins were differentially expressed in gingival tissues from patients with CP and G-AgP and may play vital roles in the progression of periodontal inflammation to different degrees. Our studies may provide a new direction for personalized periodontal treatment. (C) 2015 Elsevier Ltd. All rights reserved.
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