期刊
PAIN
卷 155, 期 1, 页码 168-178出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1016/j.pain.2013.09.022
关键词
mu-Opioid receptors; Development; Neonate; Periaqueductal grey; Dorsal horn
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- BBSRC strategic skills award
- Biotechnology and Biological Sciences Research Council [BB/I001565/1] Funding Source: researchfish
- BBSRC [BB/I001565/1] Funding Source: UKRI
Significant opioid-dependent changes occur during the fourth postnatal week in supraspinal sites (rostroventral medulla [RVM], periaqueductal grey [PAG]) that are involved in the descending control of spinal excitability via the dorsal horn (DH). Here we report developmentally regulated changes in the opioidergic signalling within the PAG and DH, which further increase our understanding of pain processing during early life. Microinjection of the mu-opioid receptor (MOR) agonist DAMGO (30 ng) into the PAG of Sprague-Dawley rats increased spinal excitability and lowered mechanical threshold to noxious stimuli in postnatal day (P)21 rats, but had inhibitory effects in adults and lacked efficacy in P10 pups. A tonic opioidergic tone within the PAG was revealed in adult rats by intra-PAG microinjection of CTOP (120 ng, MOR antagonist), which lowered mechanical thresholds and increased spinal reflex excitability. Spinal adminstration of DAMGO inhibited spinal excitability in all ages, yet the magnitude of this was greater in younger animals than in adults. The expression of MOR and related peptides were also investigated using TaqMan real-time polymerase chain reaction and immunohistochemistry. We found that pro-opiomelanocortin peaked at P21 in the ventral PAG, and MOR increased significantly in the DH as the animals aged. Enkephalin mRNA transcripts preceded the increase in enkephalin immunoreactive fibres in the superficial dorsal horn from P21 onwards. These results illustrate that profound differences in the endogenous opioidergic signalling system occur throughout postnatal development. (C) 2013 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.
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