期刊
PAIN
卷 154, 期 1, 页码 160-174出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1016/j.pain.2012.10.009
关键词
CB1 receptor; CB2 receptor; Joint pain; MIA; Opioid receptors
资金
- Spanish Ministerio de Ciencia e Innovacion [SAF2007-64062]
- Instituto de Salud Carlos III (RETICS-Red de Trastornos Adictivos-Redes Tematicas de Investigacion Cooperativa en Salud) [RD06/0001/0001, RD06/0001/1004]
- Plan Nacional sobre Drogas [PNSD 2009/026]
- Catalan Government [SGR2009-00131]
- ICREA Foundation (ICREA Academia)
- DG Research of the European Commission [LSHM-CT-2004-05166, LSHM-CT-2007-037669]
- CENIT program [CEN-20061005]
- Centro para el Desarollo Technologico Industrial
- Spanish Ministry of Science and Innovation
- Spanish Ministry of Education
- RETICS
- FEDER funds (EU)
Joint pain is a common clinical problem for which both inflammatory and degenerative joint diseases are major causes. The purpose of this study was to investigate the role of CB1 and CB2 cannabinoid receptors in the behavioral, histological, and neurochemical alterations associated with joint pain. The murine model of monosodium iodoacetate (MIA) was used to induce joint pain in knockout mice for CB1 (CB1KO) and CB2 cannabinoid receptors (CB2KO) and transgenic mice overexpressing CB2 receptors (CB2xP). In addition, we evaluated the changes induced by MIA in gene expression of CB1 and CB2 cannabinoid receptors and mu-, delta- and kappa-opioid receptors in the lumbar spinal cord of these mice. Wild-type mice, as well as CB1KO, CB2KO, and CB2xP mice, developed mechanical allodynia in the ipsilateral paw after MIA intra-articular injection. CB1KO and CB2KO demonstrated similar levels of mechanical allodynia of that observed in wild-type mice in the ipsilateral paw, whereas allodynia was significantly attenuated in CB2xP. Interestingly, CB2KO displayed a contralateral mirror image of pain developing mechanical allodynia also in the contralateral paw. All mouse lines developed similar histological changes after MIA intra-articular injection. Nevertheless, MIA intra-articular injection produced specific changes in the expression of cannabinoid and opioid receptor genes in lumbar spinal cord sections that were further modulated by the genetic alteration of the cannabinoid receptor system. These results revealed that CB2 receptor plays a predominant role in the control of joint pain manifestations and is involved in the adaptive changes induced in the opioid system under this pain state. (C) 2012 International Association for the Study of Pain. Published by Elsevier B. V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据