期刊
PAIN
卷 148, 期 2, 页码 309-319出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1016/j.pain.2009.11.017
关键词
c-Jun-N-terminal kinase; MAP kinase; Astrocytes; Spinal cord; Contralateral pain; Inflammation; Tactile allodynia
资金
- NIH [DE17794, NS54932, NS67686, TW7180]
- National Natural Science Foundation of China (NSFC) [30500153]
- National Science Council of Taiwan [NSC97-2314-B-341-002-MY3]
- Cheng Kung University Medical College, Taiwan
Peripheral inflammation induces persistent central sensitization characterized by mechanical allodynia and heat hyperalgesia that are mediated by distinct mechanisms. Compared to well-demonstrated mechanisms of heat hyperalgesia, mechanisms underlying the development of mechanical allodynia and contralateral pain are incompletely known. In this study, we investigated the distinct role of spinal JNK in heat hyperalgesia, mechanical allodynia, and contralateral pain in an inflammatory pain model. Intraplantar injection of complete Freund's adjuvant (CFA) induced bilateral mechanical allodynia but unilateral heat hyperalgesia. CFA also induced a bilateral activation (phosphorylation) of JNK in the spinal cord, and the phospho JNK1 (pJNK1) levels were much higher than that of pJNK2. Notably, both pJNK and JNK1 were expressed in GFAP-positive astrocytes. Intrathecal infusion of a selective peptide inhibitor of JNK, D-JNKI-1, starting before inflammation via an osmotic pump, reduced CFA-induced mechanical allodynia in the maintenance phase but had no effect on CFA-induced heat hyperalgesia. A bolus intrathecal injection of D-JNKI-1 or SP600126, a small molecule inhibitor of JNK also reversed mechanical allodynia bilaterally. In contrast, peripheral (intraplantar) administration of D-JNKI-1 reduced the induction of CFA-induced heat hyperalgesia but did not change mechanical allodynia. Finally, CFA-induced bilateral mechanical allodynia was attenuated in mice lacking JNK1 but not JNK2. Taken together, our data suggest that spinal JNK, in particular JNK1 plays an important role in the maintenance of persistent inflammatory pain. Our findings also reveal a unique role of JNK1 and astrocyte network in regulating tactile allodynia and contralateral pain. Crown Copyright (C) 2009 Published by Elsevier B. V. on behalf of International Association for the Study of Pain. All rights reserved.
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