期刊
PAIN
卷 138, 期 3, 页码 604-616出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.pain.2008.02.022
关键词
TRPV1; AKAP; trigeminal; pain; PKA; hyperalgesia
资金
- NIH NIDCR [DE016500, DE13942]
- NIH NIDA [DA19585]
Certain phosphorylation events are tightly controlled by scaffolding proteins such as A-kinase anchoring protein (AKAP). Oil nociceptive terminals, phosphorylation of transient receptor potential channel type 1 (TRPV1) results ill the sensitization to many different stimuli. contributing to the development of hyperalgesia. In this study, we investigated the functional involvement of AKAP150 in mediating sensitization of TRPV1, and found that AKAP150 is co-expressed in trigeminal ganglia (TG) neurons from rat and associates with TRPV1. Furthermore, siRNA-mediated knock-down of AKAP150 expression led to a significant reduction in PKA phosphorylation of TRPV1 in cultured TG neurons. In CHO cells, the PKA RII binding site on AKAP was necessary for PKA enhancement of TRPV1-mediated Ca2+-accumulation. In addition, AKAP150 knock-down in cultured TG neurons attenuated PKA sensitization of TRPV1 activity and in vivo administration of all AKAP atagonist significantly reduced prostaglandin E2 sensitization to thermal Stimuli. These data suggest that AKAP150 functionally regulates PKA-mediated phosphorylation/sensitization of the TRPV1 receptor. (C) 2008 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据