期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 67, 期 -, 页码 1-15出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2014.12.006
关键词
Biosensor; DNA; Enzymatic tool; Isothermal amplification; Ligase; Nanobiotechnology; Nuclease; PCR; Polymerase chain reaction; Signal amplification
资金
- National Key Instrument Developing Project of China [ZDYZ2013-1]
- National Natural Science Foundation of China [31400847, 21307154]
This review provides a comprehensive overview of current research involving the use of enzymatic tools for nucleic acids in the fabrication of signal amplification for biosensors. The enzymes include polymerases, nucleases, helicases, and ligases. They have the capability to mimic biological signal transduction or amplification processes, which can be applied in nucleic-acid assays by smart designs. Since most of the enzymatic tools have highly efficient catalytic effects, the sensitivity of the assays can be guaranteed. Moreover, DNA can be used as a promising material for nanobiotechnology, and the nanostructures of DNA can be engineered to recognize a variety of molecules. Thus, such enzymatic tools can be largely used for biosensing. Enzymatic tools for nucleic acids may have great practical utility in cutting-edge analytical methods and protocols. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据