期刊
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
卷 65, 期 -, 页码 54-72出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2014.09.011
关键词
Biomolecular assay; Biomolecule; Extraction; Liquid-liquid microextraction; MALDI-MS; Matrix-assisted laser desorption/ionization mass spectrometry; Nanomaterial; Nanoparticle; Preconcentration; Single-drop microextraction
Within the past decade, intensive efforts have been devoted to the development of core nanomaterials (NMs) with tunable surface properties and their integration into miniaturized solvent-extraction and sample-preparation methods for extraction, enrichment and preconcentration of ultra-trace biomolecules prior to analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). We summarize the most recent bioanalytical developments that employed NMs as effective probes using solvent microextraction in preconcentration methods coupled with MALDI-MS for assaying biomolecules from complex biological samples. We classify the nanomaterial (NM)-based miniaturized extraction/preconcentration methods into four parts: (1) nanoparticle (NP)-based single-drop microextraction; (2) NP-assisted liquid-liquid microextraction; (3) NMs as affinity and concentrating probes; and, (4) NMs as probes for bacterial analysis. We particularly emphasize efforts on NM-based miniaturized extraction and sample-preparation methods coupled with MALDI-MS for bioassays. Finally, we introduce practical applications of the above approaches. (C) 2014 Elsevier B.V. All rights reserved.
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