期刊
TOXICOLOGY LETTERS
卷 232, 期 1, 页码 310-325出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2014.10.022
关键词
Zearalenone; Pig; Epithelial intestinal cell (IPEC-1); Microarray; Gene expression
类别
资金
- Romanian Ministry of Research and Technology [PNII- ID PCE 2011-3-0339/2011-2015]
The gut represents the main route of intoxication with mycotoxins. To evaluate the effect and the underlying molecular changes that occurred when the intestine is exposed to zearalenone, a Fusarium sp mycotoxin, porcine epithelial cells (IPEC-1) were treated with 10 mu M of ZEA for 24 h and analysed by microarray using Gene Spring GX v.11.5. Our results showed that 10 mu M of ZEA did not affect cell viability, but can increase the expression of toll like receptors (TLR1-10) and of certain cytokines involved in inflammation (TNF-alpha, IL-1 beta, IL-6, IL-8, MCP-1, IL-12p40, CCL20) or responsible for the recruitment of immune cells (IL-0, IL-18). Microarray results identified 190 genes significantly and differentially expressed, of which 70% were up-regulated. ZEA determined the over expression of ITGB5 gene, essential against the attachment and adhesion of ETEC to porcine jejunal cells and of TFF2 implicated in mucosal protection. An up-regulation of glutathione peroxidase enzymes (GPx6, GPx2, GPx1) was also observed. Upon ZEA challenge, genes like GTF3C4 responsible for the recruitment of polymerase III and initiation of tRNA transcription in eukaryotes and STAT5B were significantly higher induced. The up-regulation of CD97 gene and the down-regulation of tumour suppressor genes (DKK-1, PCDH11X and TC531386) demonstrates the carcinogenic potential of ZEA. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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