期刊
TOXICOLOGY IN VITRO
卷 30, 期 1, 页码 438-445出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2015.09.018
关键词
Mancozeb; Granulosa cells; Mitochondrial membrane potential; Reactive oxygen species; Energy metabolism; AKT kinase
类别
资金
- Ministero dell'Istruzione, dell'Universita e della Ricerca I(MIUR)
Background: Mancozeb (MZ) is a fungicide that belongs to the subclass of metal (Mn/Zn) ethylene-bis-dithiocarbamate pesticides. In mouse and human granulosa cells (GCs) exposed to MZ (0.01 mu g/ml), morphological modifications and significant alterations of p53 expression level in comparison with control GCs were recorded. Objectives: To investigate if MZ (0.01 mu g/ml) induces oxidative stress and alters energy metabolism in exposed mouse GCs. Results: Following fungicide exposure, GCs showed low p53 content, a depolarized mitochondrial membrane potential (Delta Psi(m)), as well as low ATP and reduced glutathione (GSH) levels associated with increased reactive oxygen species (ROS) generation. No remarkable differences on other parameters such as ATP/ADP ratio, energy charge, as well as induction of apoptosis and DNA damage were found. The activation of AKT and PDK1 kinases in MZ-treated cells was observed. Inhibition of ROS generation by the antioxidant N-acetylcysteine (NAC) restored a normal expression level of p53. Conclusions: Our results demonstrate that the low dose of MZ here used induces a mild oxidative stress in GCs, and provides evidence for the possible involvement of AKT/PKB signaling pathway in triggering adaptive and survival response. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据