期刊
TOXICOLOGY IN VITRO
卷 29, 期 8, 页码 1977-1981出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2015.08.006
关键词
Fructose; Mononuclear cells; Oxidative stress; Apoptosis; Inflammation
类别
资金
- National Council for the Development of Science and Technology (CNPq - Brazil) [305188/2012-7]
- State Agency for the Development of Science and Technology (FAPES/CNPq/Pronex) [012/2009]
- National Council for the Development of Science and Technology (CNPq Brazil) [470748/2013-3, 400484/2013-7]
Background: Measuring of oxidative stress in peripheral blood mononuclear cells is a suitable model of dietary induced systemic oxidative stress. Thus, we aimed to evaluate whether a chronic high fructose intake could induce oxidative damage in peripheral blood and bone marrow mononuclear cells of rats. Methods: Animals were randomly assigned to the following groups: Control group (standard rat chow and tap water n = 8), and Fructose group (standard rat chow and a 10% fructose solution in the drinking water n = 8). Reactive oxygen species and cytokines were measure using flow cytometry in peripheral blood and bone-marrow mononuclear cells. Apoptotic cell death and the advanced oxidation protein products (AOPP) were also determined. Results: We observed a significant increase in ROS production in peripheral blood mononuclear cells of fructose group as compared to control rats. Apoptosis and the AOPP were higher in those animals underwent high fructose intake. Serum levels of IL-6 and IL-12 were also increased after 12 weeks of high fructose intake. Conclusion: We concluded that fructose intake leads to systemic oxidative stress and pro-inflammatory condition which affect peripheral blood mononuclear cells and bone-marrow mononuclear cells viability. (C) 2015 Elsevier Ltd. All rights reserved.
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