期刊
TOXICOLOGY IN VITRO
卷 29, 期 7, 页码 1473-1481出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2015.06.003
关键词
Carbendazim; Zebrafish larvae; Oxidative stress; Apoptosis; Immunotoxicity; Endocrine disruption
类别
资金
- State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control [2010DS700124-KF1306]
- Zhejiang Academy of Agricultural Sciences
Increasing evidence have suggested deleterious effects of carbendazim on reproduction, apoptosis, immunotoxicity and endocrine disruption in mice and rats, however, the developmental toxicity of carbendazim to aquatic organisms remains obscure. In the present study, we utilized zebrafish as an environmental monitoring model to characterize the effects of carbendazim on expression of genes related to oxidative stress, apoptosis, immunotoxicity and endocrine disruption during larval development. Different trends in gene expression were observed upon exposing the larvae to 4, 20, 100, and 500 mu g/L carbendazim for 4 and 8 d. The mRNA levels of catalase, glutathione peroxidase and manganese superoxide dismutase (CAT, GPX, and Mn/SOD) were up-regulated after exposure to different concentrations of carbendazim for 4 or 8 d. The up-regulation of p53, Apaf1, Cas8 and the down-regulation of Bcl2, Mdm2, Cas3 in the apoptosis pathway, as well as the increased expression of cytokines and chemokines, including OCCL-C1C, CCL1, IL-1b, IFN, IL-8, and TNF alpha, suggested carbendazim might trigger apoptosis and immune response during zebrafish larval development. In addition, the alteration of mRNA expression of VTG, ER alpha, ER beta 1, ER beta 2, TR alpha, TR beta, Dio1, and Dio2 indicated the potential of carbendazim to induce endocrine disruption in zebrafish lame. These data suggested that carbendazim could simultaneously induce multiple responses during zebrafish larval development, and bidirectional interactions among oxidative stress, apoptosis pathway, immune and endocrine systems might be present. (C) 2015 Elsevier Ltd. All rights reserved.
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