期刊
OSTEOARTHRITIS AND CARTILAGE
卷 22, 期 9, 页码 1271-1281出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2014.07.001
关键词
DMA (dynamic mechanical analysis); Flow cytometry; Histomorphometry; Meniscus; Micro-computed tomography; Tissue engineering
资金
- Portuguese Foundation for Science and Technology, FCT through project OsteoCart [PTDC/CTM-BPC/115977/2009]
- MultiScaleHuman project in the Marie Curie Actions-Initial Training Networks [MRTN-CT-2011-289897]
- [SFRH/BD/81356/2011]
- [SSFRH/BD/64601/2009]
- [IF/00423/2012]
Objective: To overcome current limitations of Tissue Engineering (YE) strategies, deeper comprehension on meniscus biology is required. This study aims to combine biomechanical segmental analysis of fresh human meniscus tissues and its correlation with architectural and cellular characterization. Method: Morphologically intact menisci, from 44 live donors were studied after division into three radial segments. Dynamic mechanical analysis (DMA) was performed at physiological-like conditions. Microcomputed tomography (CT) analysis of freeze-dried samples assessed micro-structure. Flow cytometry, histology and histomorphometry were used for cellular study and quantification. Results: Anterior segments present significantly higher damping properties. Mid body fresh medial meniscus presents higher values of E' compared to lateral. Cyclic loads influence the viscoelastic behavior of menisci. By increasing the frequency leads to an increase in stiffness. Conversely, with increasing frequencies, the capacity to dissipate energy and damping properties initially decrease and then rise again. Age and gender directly correlate with higher E' and tan delta. Micro-CT analysis revealed that mean porosity was 55.5 (21.2-89.8)% and 64.7 (47.7-81.8)% for freeze-dried lateral and medial meniscus, respectively. Predominant cells are positive for CD44, CD73, CD90 and CD105, and lack CD31, CD34 and CD45 (present in smaller populations). Histomorphometry revealed that cellularity decreases from vascular zone 1 to zone 3. Anterior segments of lateral and medial meniscus have inferior cellularity as compared to mid body and posterior ones. Conclusion: Menisci are not uniform structures. Anterior segments have lower cellularity and higher damping. Cyclic loads influence viscoelastic characteristics. Future TE therapies should consider segmental architecture, cellularity and biomechanics of fresh tissue. (C) 2014 Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据